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agilent [2010/05/30 19:35]
dmontaner
agilent [2017/05/24 10:36] (current)
Line 1: Line 1:
 +====== Agilent ======
 +
 +[[Agilent]] is a microarray manufacturer:
 +
 +See: 
 +  * http://www.agilent.com
 +  * [[http://www.chem.agilent.com/Scripts/PCol.asp?lPage=494|Agilent DNA Microarrays]]
 +  * [[http://www.chem.agilent.com/Scripts/PDS.asp?lPage=2547|Agilent Feature Extraction Software]]
 +  * [[http://www.genomics.agilent.com/files/Manual/G4460-90025_FE_User.pdf|Agilent Feature Extraction Software User Guide]]
 +  * [[http://www.genomics.agilent.com/files/Manual/G4460-90026_FE_Reference.pdf|Agilent Feature Extraction Software Reference Guide]]
 +
 +
 +====== Two Color Platforms ======
 +
 +When analyzing [[spotted arrays]] microarray scanning software produces a picture of the glass. An **image analysis** program is needed afterwards to transform //pixel// information from the picture into an spot intensity measurement which will reflect gene expression. Through this process each spot in the array has to be recognized in the picture, that is, some //pixels// in the image have to be assigned to the spot. The light intensity of those //pixels// is summarized into a unique measurement (usually the mean) and referred to as the **[[foreground]]** measurement of the spot. There are some other //pixels// "around" the spot which are also summarized into a single value (usually the median) know as the **[[background]]** intensity of the spot. This  **[[background]]** values are used to correct for non specific binding.
  
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