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gpr [2010/05/16 12:01] dmontaner |
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| + | ====== GenePix raw data files: GPR files ====== | ||
| + | .**[[GPR]]** is the extension used by [[GenePix]] for the files containing microarray spot scanned intensities. | ||
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| + | Information about the array design characteristics such as spot location or //block layout// is generally stored by [[GenePix]] in [[GAL]] files. Most often this information is also within [[GPR]] files so, in general, [[GPR]] are self sufficient. Thus, [[Babelomics]] should not need such layout information and indeed, [[GAL]] files **cannot** be used in [[Babelomics]]. | ||
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| + | Hence, if your [[GPR]] files do not contain the array layout information you will not be able to normalize them using [[Babelomics]] normalization tools. | ||
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| + | In an experiment carried out using [[GenePix]] technology you will have one [[GPR]] file for each of the arrays or slides you hybridized. If you are doing a **one-color** hybridization the [[GPR]] file will contain information just for //one channel// signal. If you are doing a **two-color** hybridization the [[GPR]] file will contain information for //two channel// signals. | ||
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| + | In [[Babelomics]], [[GPR]] files are considered to be the [[raw data]] files of the [[GenePix]] platform. | ||
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| + | [[GPR]] files are generally **text** format files so you can open them using a text editor or an spreadsheet. | ||
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| + | [[http://www.moleculardevices.com/pages/software/gn_genepix_file_formats.html#gpr|Here]] you can find more information about [[GenePix]] [[GPR]] file format. | ||
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| + | Information about some other [[GenePix]] data files can be found [[http://www.moleculardevices.com/pages/software/gn_genepix_file_formats.html|here]]. | ||
