Activity 1

Objective: Cluster Analysis for genes.

Data: Significant genes in a transcriptomic experiment with fibroblasts

Workflow:

  1. Open the file “fibroblasts_sig.txt” in a text editor and inspect its content. How many genes and samples do we have?
  2. Upload your file to Babelomics 5.0. Go to section Expression > Clustering
  3. Cluster genes using these parameters: SOTA + Correlation coeff. (Pearson)

Questions:

  1. Could you interpret the meaning of the heat-map? Blue and red colors? Rows and columns?
  2. Do you detect any clear clusters?
  3. Would you plan any functional approach from these clustering results?