Objective: Cluster Analysis for genes.
Data: Significant genes in a transcriptomic experiment with fibroblasts
Workflow:
Open the file “fibroblasts_sig.txt” in a text editor and inspect its content. How many genes and samples do we have?
Upload your file to Babelomics 5.0. Go to section Expression > Clustering
Cluster genes using these parameters: SOTA + Correlation coeff. (Pearson)
Questions:
Could you interpret the meaning of the heat-map? Blue and red colors? Rows and columns?
Do you detect any clear clusters?
Would you plan any functional approach from these clustering results?