#Example1: solid.fastq ################################################ #1. Open FastQC program: fastqc & #2. Trim your sample based on its quality with a minimum quality threshold of 20: fastq_quality_trimmer -t 20 -i solid.fastq -o solid_t20.fastq #3. Trim the sample based on its quality with a minimum quality threshold of 28: fastq_quality_trimmer -t 28 -i solid.fastq -o solid_t28.fastq #4.Trim the sample based on its quality with a minimum quality threshold of 28, removing the reads with a length lower than 47: fastq_quality_trimmer -t 28 -l 47 -i solid.fastq -o solid_t28l47.fastq #5. Remove the reads with less than a 90% with quality above 20: fastq_quality_filter -q 20 -p 90 -i solid.fastq -o solid_q20p90.fastq #Example2: mirna.fastq ################################################ #1. Open FastQC program: fastqc & #2. Trim your sample based on its quality with a minimum quality threshold of 20: fastq_quality_trimmer -t 20 -i mirna.fastq -o mirna_t20.fastq #3. Trim the sample based on its quality with a minimum quality threshold of 28: fastq_quality_trimmer -t 28 -i mirna.fastq -o mirna_t28.fastq #4. Trim the sample based on its quality with a minimum quality threshold of 28, removing the reads with a length lower than 30: fastq_quality_trimmer -t 28 -l 30 -i mirna.fastq -o mirna_t28l30.fastq #5.Trim the sample based on its quality with a minimum quality threshold of 28, removing the reads with a length lower than 35. fastq_quality_trimmer -t 28 -l 35 -i mirna.fastq -o mirna_t28l35.fastq