Two Color Platforms

When analyzing spotted arrays microarray scanning software produces a picture of the glass. An image analysis program is needed afterwards to transform pixel information from the picture into an spot intensity measurement which will reflect gene expression. Through this process each spot in the array has to be recognized in the picture, that is, some pixels in the image have to be assigned to the spot. The light intensity of those pixels is summarized into a unique measurement (usually the mean) and referred to as the foreground measurement of the spot. There are some other pixels “around” the spot which are also summarized into a single value (usually the median) know as the background intensity of the spot. This background values are used to correct for non specific binding.

agilent.txt · Last modified: 2017/05/24 10:36 (external edit)
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